Not known Factual Statements About thr77
Not known Factual Statements About thr77
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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in many tumors, which ends up in chromosome instability, and so may perhaps add to tumorigenesis. This protein was observed to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a significant job in endorsing cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually explained. Two added splice variants were described but in depth nucleotide sequence details is not really however available.
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Blocking peptides are peptides that bind especially to the target antibody and block antibody binding. These peptide generally consists of the epitope identified through the antibody.
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This information outlines more feasible optimization of mobile and immunoassay parameters to guarantee the very best final results are...
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This information outlines additional possible optimization of cellular and immunoassay parameters to be sure the very best benefits are...
Antibodies sure to the blocking peptide now not bind towards the epitope around the concentrate on protein. By evaluating the staining with the blocked antibody compared to the antibody by itself, one can see which staining is particular.
Target Details Cyclin E belongs for the very conserved cyclin spouse and children, whose members are characterized by a dramatic periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Distinct cyclins show unique expression and degradation designs which lead on the temporal coordination of each mitotic event. Cyclin E varieties a posh with and capabilities for a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S transition. This protein accumulates with the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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